| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Meyerhoeffer, Stella M (12)
|
MRSPAR |
29.98 |
29.71 |
-0.27 |
| 2 |
Adams, Grace C (12)
|
MRRAC |
29.89 |
30.30 |
0.41 |
| 3 |
Alexander, Sinceraty D (12)
|
MRSPAR |
32.06 |
31.92 |
-0.14 |
| 4 |
Desiderio, Sarah P (12)
|
MRUNSP |
35.21 |
32.15 |
-3.06 |
| 5 |
Mykytenko, Elizabeth (12)
|
MRRAC |
36.57 |
34.20 |
-2.37 |
| 6 |
Bauer, Demi J (12)
|
MRSPAR |
36.53 |
35.68 |
-0.85 |
| 7 |
Wei, Alice (11)
|
MRRAC |
35.57 |
36.73 |
1.16 |
| 8 |
Caruvana, Lauren E (11)
|
MRRAC |
39.54 |
38.22 |
-1.32 |
| 9 |
DiMarco, AnnaLynn (11)
|
MRSPAR |
39.50 |
38.44 |
-1.06 |
| 10 |
Alfaro, Leslie (12)
|
MRRAC |
49.15 |
40.83 |
-8.32 |
| 11 |
Stromski, Madison L (12)
|
MRIA |
38.53 |
41.78 |
3.25 |
| 12 |
Argueta, Kirsten F (11)
|
MRPAC |
44.99 |
45.67 |
0.68 |